Examples of 'nuclease activity' in a sentence
Meaning of "nuclease activity"
nuclease activity: This phrase refers to the enzymatic activity of nucleases, which are enzymes that cleave the phosphodiester bonds between nucleotide subunits in nucleic acids like DNA and RNA
How to use "nuclease activity" in a sentence
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nuclease activity
Methods using hybridization and nuclease activity.
The TaqI nuclease activity in the host strain was tested.
All reagents are most preferably free of all nuclease activity.
Nuclease activity assays.
Fractions were assayed for nuclease activity as described above.
The chaotropic agent stabilizes nucleic acids by inhibiting nuclease activity.
Nuclease activity in the tear was quantitated using a DNase detection kit assay FIG.
Other suitable proteases lacking nuclease activity can be used.
In some cases, the enzymatic activity of the chimeric site-directed modifying polypeptide is nuclease activity.
Exemplary DNA binding proteins having nuclease activity function to nick or cut double stranded DNA.
This is with a view to providing as equal as possible protection from nuclease activity.
When all four positions are mismatched, nuclease activity is no longer detectable.
The IPC nucleic acid molecule is modified so as to protect it from nuclease activity.
Signal is generated by 5 ′ nuclease activity of polymerase when it extends the second primer.
The methods of the invention may comprise a step of inactivating nuclease activity in the sample.
See also
In preferred embodiments, the enzymes with 5 ' nuclease activity are thermostable and thermoactive nucleic acid polymerases.
The inserted domain may be comprise a mutation that reduces the nuclease activity of the domain.
In some cases, the nuclease activity introduces a double strand break in the target DNA.
RecA is a recombination protein with protease and nuclease activity.
In short, the conventional method uses a nuclease activity different from the present method.
In some embodiments, the functional domain comprises nuclease activity.
For each TALEN that gave positive results, nuclease activity was specific to the cognate target.
For optimal results, all reagents should be free of nuclease activity.
Nuclease activity toward DNA in vitro, and.
A number of assays have been developed that utilize the nuclease activity of a DNA polymerase.
The nuclease activity of Cas9 cleaves target DNA to produce double strand breaks.
Therefore, a polymerase having nuclease activity may be used.
In this embodiment, a part or whole of the enriching primer is degradable by a nuclease activity.
In other embodiments, ZFPs with nuclease activity are designed.
Generation of polymerase mutants with reduced polymerase activity but unaltered 5 ' nuclease activity.
Preferably the DNase has minimal nuclease activity towards the DNA strand of a DNA, RNA duplex.
In some cases, the enzymatic activity is nuclease activity.
The predominant serum nuclease activity is known to be a 3 ′ exonuclease.
Additionally, many DNAPs are known to have nuclease activity.
As discussed, on-target nuclease activity can be increased or decreased.
The cleavage means is not restricted to enzymes having solely 5 ' nuclease activity.
Thus, the 5 ' nuclease activity can be uncoupled from polymerization.
The assays to determine synthetic activity and 5 ' nuclease activity are described below.
Also, off-target nuclease activity can be increased or decreased.
These results indicate that RNAi ablates target mRNAs through the generation of a sequence-specific nuclease activity.
Moreover, these cytotoxic molecules exhibit a nuclease activity toward DNA in vitro.
The nuclease activity can be determined by light absorbance at 260 nm.
In further embodiments, the IPC is modified so as to protect it from nuclease activity.
The main nuclease activity in the blood origins from 3 ' - exonucleases.
Thus, there is an advantage in protecting the IPC from nuclease activity.
Hybridized probes are cleaved by the 5 ' to 3 ' nuclease activity of the polymerase during primer extension.
In another aspect, step ( b ) does not include the use of an enzyme having a nuclease activity.
This will be consistent with the nuclease activity ( RNase or DNase ).
In an embodiment of this invention, the kit further comprises an enzyme having a 5 ' nuclease activity.
Preferred enzymes with 5 ' nuclease activity include template-dependent nucleic acid polymerases.
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Examples of using Nuclease
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Brackets indicate the nuclease digestion products
The nuclease bands are indicated with arrows
Phosphorothioate ODNs are primarily resistant to serum nuclease
