Examples of 'nuclease domain' in a sentence
Meaning of "nuclease domain"
nuclease domain: A specific region within a protein responsible for nuclease activity, involving the cleavage of nucleic acids
How to use "nuclease domain" in a sentence
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nuclease domain
The mutation can occur in the nuclease domain of the endoribonuclease.
A nuclease domain possesses catalytic activity for nucleic acid cleavage.
All of the Cas proteins contain a conserved a RuvC nuclease domain irrespective of sequence diversity.
In another embodiment, the linker connects a DNA-binding domain to a nuclease domain.
In some embodiments, the inserted nuclease domain comprises a RuvC domain.
In another embodiment, the nucleic acid comprises a nucleotide sequence encoding a nuclease domain.
In some aspects, the inserted nuclease domain comprises a RuvC domain.
In other embodiments, the protein comprises a nuclease domain.
In some aspects, the engineered nuclease domain comprises mutation of a conserved histidine.
In one embodiment, the protein comprises a nuclease domain.
In some embodiments, the engineered nuclease domain comprises mutation of a conserved aspartic acid.
In some embodiments, the hybrid nuclease-albumin molecule further includes a second nuclease domain.
In a preferred embodiment, the other putative nuclease domain is a HincII-like endonuclease domain.
The modified site-directed polypeptide comprises an inserted HNH or RuvC nuclease domain.
In some aspects, the inserted nuclease domain is in tandem to the first nuclease domain.
See also
They contain one transmembrane domain, a catalytic phosphodiesterase domain and a C-terminal nuclease domain.
In some aspects, the cleaving is performed by a nuclease domain of the site-directed polypeptide.
Similar results were obtained using ZFPs fused to the Fokl nuclease domain ( not shown ).
In a specific embodiment, the nuclease domain in a zinc finger nuclease is Fokl.
The site-directed polypeptide 405 can comprise a nuclease domain.
In some embodiments, the inserted nuclease domain comprises a HNH domain.
In some aspects, the nuclease domain of the site-directed polypeptide 405 can be enzymatically inactive.
In some instances, the modification occurs in the nuclease domain of an endoribonuclease.
In some embodiments, the inserted nuclease domain is C-terminal to the second nuclease domain.
Site-directed polypeptides that comprise one substantially inactive nuclease domain can be referred to as nickases.
In some aspects, the modified nuclease domain comprises an HNH nuclease domain.
In a preferred embodiment a nuclease domain comprises Fok1.
In some embodiments, the inserted nuclease domain is C-terminal to the first nuclease domain.
In another disclosure, nucleotides encoding for the nuclease domain of CRISPR Cas9 complex are provided.
For example, a primate Fc domain or nuclease domain may be included in the subject sequence.
The CRISPR / Cas nuclease also comprises at least one nuclease domain having endonuclease activity.
In some aspects, the inserted nuclease domain is N-terminal to the second nuclease domain.
For example, a Cas9 protein can comprise a RuvC-like nuclease domain and a HNH-like nuclease domain.
In some aspects, the inserted nuclease domain is adapted to cleave a DNA in a DNA-RNA hybrid.
In some aspects, the inserted nuclease domain comprises a HNH domain.
In some embodiments, the inserted nuclease domain is N-terminal to the first nuclease domain.
In the embodiments, the first nuclease domain is an RNase, for example, human RNasel.
In some aspects, the engineered nuclease domain comprises a D10A mutation.
In some embodiments, the engineered nuclease domain comprises a H840A mutation.
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Examples of using Nuclease
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Brackets indicate the nuclease digestion products
The nuclease bands are indicated with arrows
Phosphorothioate ODNs are primarily resistant to serum nuclease
