Examples of 'packaging cell' in a sentence
Meaning of "packaging cell"
packaging cell - This phrase can have different meanings depending on the context. In biotechnology or cell culture, a packaging cell refers to a genetically modified cell used to produce viral vectors for gene therapy. In the context of product or goods packaging, it may refer to a specific type of cell or compartment in a packaging material or container
How to use "packaging cell" in a sentence
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packaging cell
Such a cell is usually called a packaging cell.
Alternatively the packaging cell line harbours a provirus.
This embodiment further comprises a packaging cell.
Alternatively the packaging cell line harbors a provirus.
The vectors are then introduced into the packaging cell.
Culturing a stable packaging cell line as described above.
The envelope protein is provided by the packaging cell line.
Various retroviral packaging cell lines are also based on these cells.
Generation of tTA expressing packaging cell lines.
The packaging cell and the cell culture media are collected.
A vector is transduced in a packaging cell by a means known in the art.
This embodiment of the invention further comprises a packaging cell.
Inserting in the stable packaging cell line a transfer vector.
A recombinant virus vector is introduced into a suitable packaging cell.
Packaging cell lines are used to prepare the retroviral vector particles.
See also
A number of attempts have been made to establish packaging cell systems.
Lentiviral packaging cell lines are available and known generally in the art.
The above constructs are separately transfected into a packaging cell line.
A packaging cell will generally be needed in order to produce sufficient amount of adenoviruses.
Replication of the vector requires growth in the packaging cell line.
Supernatants resulting from the packaging cell line are collected at various times.
These proteins must be provided in trans by a helper or packaging cell line.
Packaging cell lines for retroviral vectors are known to those of skill in the art.
Any suitable cell line can be used as a packaging cell line.
The packaging cell line may stably express the necessary viral proteins.
As a result packaging defective vectors and packaging cell lines were developed.
The packaging cell line is then infected with a helper virus such as adenovirus.
This construct is then placed into a packaging cell as described above.
A packaging cell is used to form a virus particle that is capable of infecting a target cell.
These viruses can be produced only in viral packaging cell lines.
She had retrovirus packaging cell gene therapy combined with tumour resection.
Stable cell clones are used for the generation of packaging cell lines.
An alternative to stably transfected packaging cell lines is to use transient transfected cell lines.
The retroviral vector may be transfected into a retroviral packaging cell line.
The cells used to produce the packaging cell line may be human cells.
These functions are therefore integrated in different loci of the packaging cell genome.
Fresh aliquots of frozen packaging cell lines were thawed for use in each experiment.
Transient transfection has numerous advantages over the packaging cell method.
The packaging cell is transfected with the minimal vector construct to make a producer cell.
Consequently efforts have been directed at improving the safety of packaging cell constructs.
The choice of the packaging cell line determines the cells that will be targeted.
The missing viral functions are supplied in trans by the packaging cell line.
The packaging cell line produces viral particles that contain the vector genome.
The vectors are introduced vía transfection or infection into the packaging cell line.
The retroviral packaging cell line is preferably in the form of a transiently transfected cell line.
All of these deleted functions can be expressed in the packaging cell line.
The retroviral vector can be produced in a packaging cell line containing a helper plasmid or plasmids.
Such RNAs are optionally stably transformed into the packaging cell line.
The packaging cell may be a cell cultured in vitro such as a tissue culture cell line.
Producer cells derived from human amphotropic and xenotropic packaging cell lines.
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