Examples of 'packaging cell line' in a sentence
Meaning of "packaging cell line"
Packaging cell line: Refers to a production line or process in manufacturing that specifically deals with packaging and labeling products before distribution
How to use "packaging cell line" in a sentence
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packaging cell line
Alternatively the packaging cell line harbours a provirus.
The envelope protein is provided by the packaging cell line.
Alternatively the packaging cell line harbors a provirus.
The above constructs are separately transfected into a packaging cell line.
Culturing a stable packaging cell line as described above.
Replication of the vector requires growth in the packaging cell line.
Inserting in the stable packaging cell line a transfer vector.
These proteins must be provided in trans by a helper or packaging cell line.
Supernatants resulting from the packaging cell line are collected at various times.
The retroviral vector may be transfected into a retroviral packaging cell line.
The cells used to produce the packaging cell line may be human cells.
The missing viral functions are supplied in trans by the packaging cell line.
The packaging cell line may stably express the necessary viral proteins.
Any suitable cell line can be used as a packaging cell line.
The packaging cell line is then infected with a helper virus such as adenovirus.
See also
The vectors are introduced vÃa transfection or infection into the packaging cell line.
The choice of the packaging cell line determines the cells that will be targeted.
All of these deleted functions can be expressed in the packaging cell line.
The packaging cell line produces viral particles that contain the vector genome.
Such RNAs are optionally stably transformed into the packaging cell line.
The retroviral packaging cell line is preferably in the form of a transiently transfected cell line.
The present invention provides a method to obtain a stable lentiviral packaging cell line.
The retroviral vector can be produced in a packaging cell line containing a helper plasmid or plasmids.
This can increase the titer of virus recovered from the packaging cell line.
Increasing the vector copies in the packaging cell line should also im prove titers.
This may increase the titer of virus recovered from the packaging cell line.
The vector construct is introduced into a packaging cell line which will generate infectious virions.
The packaging cell line Phoenix A was transfected with these vectors using calcium phosphate.
The vector then is employed to transduce a packaging cell line to form a producer cell line.
After insertion, some viral genomes can be packaged in vitro or using a packaging cell line.
Alternatively, the packaging cell line harbors an integrated provirus.
Such a cell line is usually termed as AAV packaging cell line.
Pharmaceutical composition comprising a packaging cell line according to any of the preceding claims 1 to 9.
To generate the packaged vector, the vector plasmid is transfected into the packaging cell line.
Particularly, 3rd generation stable packaging cell line is obtained by further integrating desired env protein.
To generate the packaged vector, the retroviral vector is transfected into the packaging cell line.
Supernatant obtained from the packaging cell line contains the desired BIV-based lentiviral vector.
In a particular embodiment, the cells used to produce the packaging cell line are human cells.
A Lentiviral packaging cell line comprising,.
In a particular examples, the cells used to produce the packaging cell line are human cells.
Packaging Cell Line Transduction Packaging cell line transduction is performed as described in Example 2Bv.
Producer cell lines are named by noting the packaging cell line and inserted vector.
A packaging cell line producible by a method according to any one of claims 6 to 10.
A retrovirus vector system basically consists of two elements, a packaging cell line and a vector genome.
In a preferred embodiment the packaging cell line of the invention includes the VSV-G env gene.
To generate a recombinant retroviral particle, the retroviral vector is transfected into the packaging cell line.
In a particular preferred embodiment, the packaging cell line is made from human cells, e . g.
The present invention provides a new strategy to generate an HIV-1-based packaging cell line.
Alternatively, the packaging cell line can lack sequences encoding a viral envelope ( env ) protein.
Figure 2 shows a map of plasmid pVpr-RTIN used in the packaging cell line of the invention.
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