Examples of 'packaging cell lines' in a sentence
Meaning of "packaging cell lines"
Packaging cell lines: The process of preparing and storing cell cultures in containers or vials for transportation, preservation, or research purposes. It involves carefully handling and sealing cell lines to maintain their viability and integrity
How to use "packaging cell lines" in a sentence
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packaging cell lines
Generation of tTA expressing packaging cell lines.
Various retroviral packaging cell lines are also based on these cells.
These viruses can be produced only in viral packaging cell lines.
Packaging cell lines are used to prepare the retroviral vector particles.
As a result packaging defective vectors and packaging cell lines were developed.
Lentiviral packaging cell lines are available and known generally in the art.
Stable cell clones are used for the generation of packaging cell lines.
Packaging cell lines for retroviral vectors are known to those of skill in the art.
An alternative to stably transfected packaging cell lines is to use transient transfected cell lines.
Producer cells derived from human amphotropic and xenotropic packaging cell lines.
Fresh aliquots of frozen packaging cell lines were thawed for use in each experiment.
These retroviral vectors are especially suited for use in certain packaging cell lines.
The recombinant preparation of particularly suitable packaging cell lines is likewise a constituent of this invention.
There are also disclosed herein methods of producing viral replicon particles using packaging cell lines.
A particular problem is the lack of suitable packaging cell lines for these other serotypes.
See also
New packaging cell lines derived from PER.
This has severely limited the application and the development of packaging cell lines for rAAV.
Amphotropic and ecotropic packaging cell lines producing RFEL were also selected for etoposide resistance.
And RNA virions grown on packaging cell lines.
Two other packaging cell lines are known as Psi CRIP and Psi CRE.
In this respect, conventional methods are based on the use of packaging cell lines.
Preferably, the packaging cell lines are third generation packaging cell lines.
Figure 18 is a graph depicting luciferase vector packaging by different alphavirus packaging cell lines.
Preferably, the packaging cell lines are second generation packaging cell lines.
These vRNAs are packaged into virus-like particles in the packaging cell lines.
Packaging cell lines can express ecotrophic, amphotropic or xenotropic envelope gene products.
In preferred embodiments, stable alphavirus packaging cell lines are utilized for replicon particle production.
Such packaging cell lines provide the necessary retroviral gag, pol and env proteins in trans.
Those viral vectors lack sequences homologous to E1 sequences in said packaging cell lines.
Alternatively, packaging cell lines have been constructed in order to avoid the inefficient transfection step.
From the first intermediate, 2nd and 3rd generation stable packaging cell lines can be obtained.
These can be generated in packaging cell lines that produce only replication-defective viruses.
Example 16 Generation of tTA expressing packaging cell lines A.
In some embodiments, stable alphavirus packaging cell lines are used to produce recombinant alphavirus particles.
Packaging cell lines for Ad5 vectors typically comprises E1 encoded proteins derived from adenovirus serotype 5.
Suitable packaging cell lines for adenoviruses with a deletion in the E1 region include, for example, PER.
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