Examples of 'packaging cells' in a sentence
Meaning of "packaging cells"
packaging cells: Refers to the process of enclosing or bundling individual cells or items together for storage, transport, or organization
How to use "packaging cells" in a sentence
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packaging cells
Appropriate packaging cells may also be used.
This transcomplementation step can be performed passing through packaging cells.
Other packaging cells will be used if requested.
It may also be a recombinant virus used to infect the packaging cells.
Suitable packaging cells are discussed in more detail below.
Preferred cell lines for use as packaging cells are insect cell lines.
Tunicamycin treatment relieves the block to superinfection of the parental packaging cells.
Packaging vectors and packaging cells are as described hereinabove.
Packaging cells are used to form virus particles that are capable of infecting a host cell.
The invention thus provides packaging cells that produce infectious rAAV.
These infectious supernatants are then used to transduce a fresh monolayer of alphavirus packaging cells.
Construction of packaging cells for retroviral vectors is a tedious procedure.
Any suitable cell line can be employed to prepare packaging cells of the invention.
The infected packaging cells are then incubated for three days.
Any suitable cell line can be employed to prepare packaging cells of the disclosure.
See also
The resulting packaging cells produce infectious virus particles containing the gene of interest.
Use of viral supernatant versus coculture on packaging cells producing the virus continuously.
The filtered supernatants are then used to transduce a fresh monolayer of Sindbis packaging cells.
Also provided are recombinant packaging cells obtained by the method described above.
The filtered supernatants are then used to infect a fresh monolayer of Sindbis packaging cells.
The cell culture media and the packaging cells can be pooled or collected separately.
Figure 5 depicts vRNA transcripts in packaging cells.
The vectors may transduce the packaging cells through any means known in the art.
Because of these modifications, viral production is generally accomplished using retrovirus packaging cells.
Infectious virus particles may be collected from the packaging cells using conventional techniques.
In step a, suitable packaging cells depend on the type of vaccinia virus to be amplified.
Viruses can be generated by a double homologous recombination in packaging cells described in patent No.
For example, where the packaging cells are insect cells, baculovirus may serve as a helper virus.
Generation of " producer cells " is accomplished by introducing retroviral vectors into the packaging cells.
The virus stock released from the packaging cells thus contains only recombinant virus.
Presently, subcloning and testing of individual subclones is applied to isolate suitable packaging cells.
This includes packaging cells such as PER.
This step leads to the liberation of poxvirus ( in particular vaccinia virus ) from packaging cells.
Remove the virus-producing packaging cells from the incubator.
GFP-pBABE plasmids were transiently transfected into Pheonix packaging cells.
Therefore, the virus stock released from the packaging cells contains only recombinant virus.
C6 packaging cells to very high titers.
Preferably, the core additional comprises a matrix, the packaging cells being immobilized by the matrix.
Thus, packaging cells allow VRPs to act as self-propagating viruses.
Preferably, the core additionally comprises a matrix, the packaging cells being immobilized by the matrix.
Adenovirus packaging cells from monkey origin that are able to trans-complement E1-defective recombinant adenoviruses.
Over time, replicon particles can be harvested from packaging cells infected with the seed stock.
The cells designed to be traps are, for example, co-cultured directly with the packaging cells.
One day before lipofection 3x105 retroviral packaging cells are seeded into 6cm petri or culture dishes.
This vector was stably transduced into PG13 packaging cells.
C6 packaging cells with an Ad35 adapter plasmid.
FIG . 5 depicts vRNA transcripts in packaging cells.
Amphotropic Phoenix packaging cells were transfected with hOX40-MSCV plasmid vía Fugene HD ( Roche ).
VSV-G pseudotyped vector can be produced by expressing VSV-G in packaging cells.
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Examples of using Packaging
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Put the packaging in applicable containers to recycle it
Remove all paper packaging and foil
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