Examples of 'phenyl sepharose' in a sentence
Meaning of "phenyl sepharose"
phenyl sepharose: Refers to a type of chromatography resin commonly used in biochemistry and molecular biology for protein purification processes
How to use "phenyl sepharose" in a sentence
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phenyl sepharose
The mixture was applied onto the phenyl sepharose column.
On phenyl Sepharose chromatography the hydrogenase exhibited hydrophobic properties.
The appropriate hydrophobic substance preferably comprises phenyl Sepharose.
Phenyl sepharose HP has a strong hydrophobic property and excellent purification capability.
Enzymatically active fractions were pooled and loaded onto a Phenyl Sepharose column.
The Phenyl Sepharose step was performed at room temperature.
Any suitable resin may be used, for example phenyl sepharose or octyl sepharose.
Phenyl sepharose HP is preferred.
Lane 2 is an aliquot of the pooled activity peak eluted from the phenyl sepharose fractionation.
Thus Phenyl sepharose HP is preferably used as the media for hydrophobic chromatography . Examples.
Purified with the aid of well-known absorption methods, such as phenyl sepharose absorption techniques.
Superdex The pooled Phenyl Sepharose fractions are then subjected to size exclusion chromatography, using Superdex 75.
Purified with the aid of well-known adsorption methods, such as phenyl sepharose adsorption techniques.
The characteristics of Phenyl Sepharose 6 FF ( available from GE Healthcare ) are given below.
The method according to claim 6, wherein the hydrophobic chromatography is performed on Phenyl sepharose HP.
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Fraction IIb was loaded onto the phenyl sepharose column at 78 ml / hr.
The bispecific molecules were further purified by hydrophobic interaction chromatography ( HIC ) on a Phenyl Sepharose column.
Phenyl Sepharose 6 FF ( available from GE Healthcare ) is a highly cross-linked derivated based on agarose.
The experiment was repeated using a phenyl sepharose column, Materials.
In addition, the presence of 0.3 M ammonium sulfate promotes hydrophobic interaction with a phenyl sepharose column.
This provided conditions suitable for collagenase binding to Phenyl Sepharose FF ( low sub ) media.
The contaminants were removed by adsorption with phenyl Sepharose 4B.
The hydrophobic interaction column of step ( b ) is a phenyl sepharose column ; or.
A preferred hydrophobic interaction resin is phenyl sepharose CL-4B.
The sharp, earlier eluting peak retains its homogeneity when subjected to phenyl Sepharose CL-4B chromatography.
The sample was centrifuged and the supernatant was loaded on a phenyl sepharose column ( 150 ml ).
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Examples of using Sepharose
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In one embodiment the solid support is sepharose
Sepharose has been found to be suitable for this purpose
Enzyme was purified by means of a glutathione sepharose column
Examples of using Phenyl
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Methyl salicylate and phenyl salicylate salol
Phenylacetylene is an alkyne hydrocarbon containing a phenyl group
Particularly preferred is a phenyl group or a tolyl group
