Examples of 'restriction endonuclease sites' in a sentence
Meaning of "restriction endonuclease sites"
restriction endonuclease sites - are specific DNA sequences recognized by restriction enzymes, which are proteins that cleave DNA at or near those recognition sequences. These sites are commonly used in molecular biology for DNA manipulation and gene cloning
How to use "restriction endonuclease sites" in a sentence
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restriction endonuclease sites
Relevant restriction endonuclease sites are also shown.
Thin vertical lines indicate constant restriction endonuclease sites.
Selected restriction endonuclease sites arc marked.
Polymerase chain reaction will be performed to amplify the DNA with certain restriction endonuclease sites.
The restriction endonuclease sites were confirmed by digestion.
These oligonucleotides contain unique restriction endonuclease sites needed for the assembly.
Restriction endonuclease sites can be incorporated into the primers.
For instance they may introduce or remove restriction endonuclease sites or alter codon usage.
The restriction endonuclease sites used for cloning are boxed.
The locations of 6 basepair restriction endonuclease sites are noted.
Restriction endonuclease sites are located on the DNA as shown.
Alternatively, the primers optionally incorporate restriction endonuclease sites.
Important restriction endonuclease sites are indicated.
Conventionally, this could be done by incorporation of restriction endonuclease sites into the DNA template.
Selected restriction endonuclease sites are marked.
See also
Vertical arrows indicate polymorphic ( C / T ) sequences giving rise to polymorphic restriction endonuclease sites.
The predetermined restriction endonuclease sites in the modules are unique within the module.
Figure 15A is a physical map showing restriction endonuclease sites of plasmid pSuFel.
Additional restriction endonuclease sites were also incorporated into PCR primers to facilitate subsequent cloning.
In this way, plasmid pNPI121 with these restriction endonuclease sites eliminated was obtained.
Restriction endonuclease sites and Kozak consensus region which were supplied by synthetic oligonucleotides are indicated.
Other methods have employed restriction endonuclease sites to separate a donor / acceptor pair.
Restriction endonuclease sites flanking the Bst XI cloning sites are shown.
Finally, all but one remaining restriction endonuclease sites were removed in every gene sequence.
Restriction endonuclease sites are also shown, for example, in Figure 2.
Figure 15D is a physical map showing restriction endonuclease sites of plasmid pSuFe4.
The restriction endonuclease sites needed for cloning are introduced vÃa the following PCR primers.
Each 5 ' and 3 ' PCR primer contains artificial restriction endonuclease sites for cloning purposes.
No Ear I restriction endonuclease sites such that insertion of sequences would produce a unique site.
Figure 2 shows examples of restriction endonuclease sites for Hpa I ;.
Restriction endonuclease sites used for Maxam-Gilbert DNA sequence analysis of the 1.1 kb cDNA are shown.
Figure 10A is a physical map showing restriction endonuclease sites of pSU17.
Abbreviations for restriction endonuclease sites in the drawings are as follows,.
Figure 16B is a physical map showing restriction endonuclease sites of plasmid pCS325.
There must be unique restriction endonuclease sites on each side of the identified mutation site ( s ).
Figure 10F is a physical map showing restriction endonuclease sites of pCab-SU13.
Fig . 2 shows the restriction endonuclease sites of the pag C locus.
Figure 10B is a physical map showing restriction endonuclease sites of pSSU-SU11.
The Nco I and Sfi I restriction endonuclease sites at 5 ' and 3 ' were included in the synthesized fragments.
Figure 16A is a physical map showing restriction endonuclease sites of plasmid pPAT108.
The primers may also carry restriction endonuclease sites for use in cloning the amplified DNA.
Figure 25A is a physical map showing restriction endonuclease sites of plasmid pZ6A-SU1.
For example, appropriate restriction endonuclease sites can be engineered ( e.g., using PCR ).
Figure 25B is a physical map showing restriction endonuclease Sites of plasmid pZ6AT-SU1.
For example, by using the indicated restriction endonuclease sites flanking the promoter, R.B.S. e . g.
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Examples of using Endonuclease
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Homing endonuclease recognition sites are extremely rare
Preferably the enzyme is a homing endonuclease or a meganuclease
The endonuclease enzyme restriction sites are underlined
