Examples of 'restriction endonucleases' in a sentence
Meaning of "restriction endonucleases"
Restriction endonucleases, also known as restriction enzymes, are enzymes found in bacteria that can cut DNA molecules at specific sequences. They are used extensively in biotechnology for DNA manipulation, such as cutting DNA into fragments for further analysis or genetic engineering purposes.
How to use "restriction endonucleases" in a sentence
Basic
Advanced
restriction endonucleases
Deletion can be effected using restriction endonucleases.
Restriction endonucleases and other enzymes.
Digest with appropriate restriction endonucleases.
For restriction endonucleases common abbreviations have been used.
Suitable methylation sensitive restriction endonucleases are described supra.
Restriction endonucleases itself are widely known in the art.
Sites of cleavage by restriction endonucleases are indicated.
Restriction endonuclease maps were prepared by using various restriction endonucleases.
Recognition sequences for restriction endonucleases are italicized for emphasis.
Fifteen legionellae isolates were studied for the presence of restriction endonucleases.
The pools are digested with restriction endonucleases to yield restriction fragments.
The vector is most conveniently cut by means of suitable restriction endonucleases.
This permits the use of restriction endonucleases in preparing the construct.
Examples of such modifying enzymes include ligases and restriction endonucleases.
Restriction endonucleases and the restriction modification system exist exclusively in prokaryotes.
See also
restriction endonuclease
restriction endonuclease sites
restriction enzyme
restriction enzyme analysis
Further disclosed are chimeric restriction endonucleases.
Restriction endonucleases play a very important role in modern molecular cloning techniques.
In situ digestion of barley chromosomes with restriction endonucleases was examined.
Additional restriction endonucleases can be tested to increase the number of polymorphisms detected.
Such processing may include the use of restriction endonucleases.
Rare cutters are restriction endonucleases that have a relatively long recognition sequence.
Differences were observed in the band patterns produced with different restriction endonucleases.
This was digested with the restriction endonucleases EcoRV and BamHl.
The degree of the deletion was investigated from a cleavage pattern by restriction endonucleases.
The sites for the restriction endonucleases EcoRI and EcoRV are indicated.
The detection of methylation may furthermore be performed using methylation specific restriction endonucleases.
Various families of restriction endonucleases are found among all three catalytic classes.
Both orientations are identified by double restriction with restriction endonucleases HindIII and PstI.
Restriction endonucleases can also be utilized effectively to detect mutations in the tagA gene.
The recognition sites for several restriction endonucleases are shown above the nucleotide sequences.
Restriction endonucleases and other enzymes were used in accordance with the manufacturers recommendations.
The jnb amplification products were subjected to rflp using restriction endonucleases drai and hinfi.
Other methylation sensitive restriction endonucleases will be known to those of skill in the art.
The plasmids isolated and purified from said clones were mapped with restriction endonucleases.
Cleavage sites for certain restriction endonucleases employed in constructing expression vectors are indicated.
In certain embodiments the tag section may comprise recognition sites for restriction endonucleases.
Suitable restriction endonucleases have only one recognition and cleavage site in the respective plasmid.
The resulting plasmid is then linearized with the restriction endonucleases HindIII and EcoRI.
Enzymes Restriction endonucleases were used according to instructions of the manufacturer.
The resultant product was then digested with the restriction endonucleases EcoRI and BglII.
Restriction endonucleases cut both strands of DNA at the same time.
The plasmid thus obtained was digested with the restriction endonucleases NruI and HindIII.
DNA restriction endonucleases are invaluable reagents for the molecular biologist.
The resultant product was then digested with the restriction endonucleases EcoRI and BgIII.
Type II restriction endonucleases are of most general utility in the method of the invention.
Any sequence might be inserted between two repeats in the cassette using suitable restriction endonucleases.
Examples of type IIS restriction endonucleases include BsmFI and FokI.
The ability to incorporate a gene sequence into a suitable vector is typically performed using restriction endonucleases.
They are most often created by restriction endonucleases when they cut DNA.
DNA can be similarly digested using a cocktail of nucleases including restriction endonucleases.
You'll also be interested in:
Examples of using Endonucleases
Show more
Endodeoxyribonuclease are both endonucleases and deoxyribonucleases
Deletion can be effected using restriction endonucleases
Such nucleases include endonucleases and exonucleases