Examples of 'restriction endonuclease' in a sentence
Meaning of "restriction endonuclease"
restriction endonuclease: Restriction endonuclease is an enzyme that recognizes specific DNA sequences and cuts the DNA at or near these sequences. It is commonly used in genetic engineering and molecular biology for DNA manipulation and analysis purposes
How to use "restriction endonuclease" in a sentence
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restriction endonuclease
A unique restriction endonuclease cleavage site was introduced.
This is usually effected by incubation with a restriction endonuclease.
In principle any restriction endonuclease can be used.
Restriction endonuclease digestion conditions and analysis.
The deletions are restriction endonuclease fragments.
Restriction endonuclease digests were performed as indicated.
The adaptor comprises a restriction endonuclease cleavage site.
The restriction endonuclease sites were confirmed by digestion.
Addition of reaction components for restriction endonuclease digestion.
Relevant restriction endonuclease sites are also shown.
A preferred restriction enzyme is a restriction endonuclease.
Selected restriction endonuclease sites arc marked.
Selected clones underwent extensive restriction endonuclease mapping.
Various restriction endonuclease cloning sites are indicated.
The resulting plasmid was analyzed by restriction endonuclease digestion.
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Methods of restriction endonuclease digestion are known to skilled person.
Several codons were changed to create restriction endonuclease cleavage sites.
Some restriction endonuclease cleavage sites are also indicated.
By digestion of a nucleic acid with a restriction endonuclease or by chemical synthesis.
Restriction endonuclease maps of members of each group are prepared.
The relative positions of restriction endonuclease recognition sites are indicated.
Restriction endonuclease cleavage sites are indicated.
Clone pBRgag thereby obtained is analysed by restriction endonuclease mapping.
Figure i shows a restriction endonuclease site map of pSRQll.
This example shows the utilization of BstUI restriction endonuclease.
Underlining indicates restriction endonuclease site introduced by oligonucleotide.
A fragment within the gene is removed using restriction endonuclease.
Method of restriction endonuclease digestion are known to skilled person.
Preferably the high temperature resistant restriction endonuclease is PspGI.
Important restriction endonuclease sites are indicated.
The template preferably has an internal restriction endonuclease site.
Selected restriction endonuclease sites are marked.
The one or more restriction enzymes may be a restriction endonuclease.
The restriction endonuclease is preferably MseI.
The modifying portion is preferably not a recombinase or a restriction endonuclease.
A restriction endonuclease map is presented.
This example shows the use of BstUI restriction endonuclease.
Restriction endonuclease sites can be incorporated into the primers.
Thin vertical lines indicate constant restriction endonuclease sites.
The restriction endonuclease sites used for cloning are boxed.
All the primers contained a restriction endonuclease site for cloning.
Restriction endonuclease map of pSRQll.
These plasmids also have been differentiated by restriction endonuclease fragment profiles.
Only a single restriction endonuclease is needed to generate a number of fragments.
Both embodiments provide strands that can be cut by a restriction endonuclease.
Restriction endonuclease maps were prepared by using various restriction endonucleases.
Preferably the restriction site is recognised by a sequence specific restriction endonuclease.
All restriction endonuclease reactions are carried out according to manufacturer instructions.
Positive colonies are grown up and plasmids are analyzed by restriction endonuclease mapping.
The SacI restriction endonuclease site within the gene is marked.
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Examples of using Endonuclease
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Homing endonuclease recognition sites are extremely rare
Preferably the enzyme is a homing endonuclease or a meganuclease
The endonuclease enzyme restriction sites are underlined
