Examples of 'sequencing primer' in a sentence
Meaning of "sequencing primer"
sequencing primer - In genetics and molecular biology, a sequencing primer is a short nucleic acid strand used to initiate DNA sequencing
How to use "sequencing primer" in a sentence
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sequencing primer
A sequencing primer can be used as an amplification primer.
The primer may be a sequencing primer.
A sequencing primer was then hybridized to the clusters.
An amplification primer may also be used as a sequencing primer.
The sequencing primer utilized is set forth below.
A kit can comprise a sequencing primer.
Adapter and sequencing primer sequences were removed from sequence reads.
In some embodiments the compositions further comprise a sequencing primer.
The choice of sequencing primer depends on the purpose of the analysis.
Optionally the label binding site is a sequencing primer site.
The location of the sequencing primer and the six positions sequenced are indicated.
Apyrase can be used to remove unreacted dNTP used to extend the sequencing primer.
The sequencing primer anneals and sequences the other end of the template.
The annealing temperature was increased and the amount of the sequencing primer was reduced.
Sequencing can be perfomed using a sequencing primer that can hybridize to a portion of the vector.
See also
The amplification product can then be sequenced using the forward and reverse sequencing primer sites.
The sequencing primer is extended with the DNA polymerase to form a sequence product.
The matrix carrying the polynucleotide fragment is suspended into a suitable solution containing the sequencing primer.
The sequencing primer was not column-purified after labelling.
For these two reasons the Excess Primer does not serve as a sequencing primer.
Thus, a sequencing primer distinct from the amplification primer is preferred.
Panel C is the control method utilizing the Excess Primer as the sequencing primer.
Subsequently, the fragments are sequenced using a sequencing primer complementary to the universal sequence.
Panel D is the control method utilizing the Limiting Primer as the sequencing primer.
The method includes a sequencing primer annealed to single-stranded DNA.
In some cases, the adaptor does not comprise a sequencing primer region.
Additionally, a T3 sequencing primer located in the pBluescript II vector was added.
In one embodiment, transposons may comprise sequencing primer binding sites.
Sequencing primer were,.
Alternatively, the binding linker may also function as the sequencing primer.
Add 4 µL of 1 mM sequencing primer to the sequencing plate.
In an embodiment of the present disclosure, the first primer is used as a sequencing primer.
On the other end the Illumina sequencing primer P7 will be used.
A set of four fluorescently labelled di-base probes compete for ligation to the sequencing primer.
A sequencing primer hybridises to the DNA, i.e. base pairs are formed.
On the basis of the thus-sequenced nucleotide sequence, another sequencing primer was synthesized.
Optionally the sequencing primer site comprises a SBS3 or SBS12 sequence.
A method as claimed in claim 1, wherein the adapter further comprises a sequencing primer site.
The mutagenic primer and the M13 sequencing primer can be chemically synthesized ( supra ).
A set of four color dye-labeled probes competes for ligation to the sequencing primer.
For example, sequencing primer DR2 can be used for sequencing the DR2 group.
The kit according to claim 8, wherein the first primer is also provided as a sequencing primer.
The universal sequencing primer has the sequence 5 ' GTAAAACGACGGCCAGT 3 '.
The binding linker may be a separate binding linker, which is not the sequencing primer.
To the tube, 12 µL of Sequencing Primer is added.
In this step, two thiolated A-nucleotides and dideoxy T-nucleotide were added to the sequencing primer.
The sequencing-enabled aptamer may additionally comprise a sequencing primer for sequencing of the aptamer.
Figure 3 is a schematic diagram showing ' step-up ' amplification of segment F for sequencing with sequencing primer f.
In the case of an M13 phage vector a universal M13 sequencing primer is preferably used.
A 3 ' tagging polynucleotide can comprise a P7 segment which can be used for annealing a sequencing primer.
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The primer includes the following modules
It was mostly gray primer and fender fill
A primer is not necessary for small repairs
Examples of using Sequencing
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Appropriate sequencing of regulatory consolidation and liberalization
There was discussion regarding the appropriate sequencing of
The process and sequencing of reform were emphasized
