Examples of 'sialyltransferase' in a sentence
Meaning of "sialyltransferase"
sialyltransferase (noun) - An enzyme that is involved in the transfer of sialic acid molecules in biological processes
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- Any of a class of enzymes that catalyze the transfer of sialic acid moieties (especially to certain glycolipids)
How to use "sialyltransferase" in a sentence
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sialyltransferase
Porcine liver sialyltransferase was purified to apparent homogeneity.
Method of producing and purifying an active soluble sialyltransferase.
Sialyltransferase polypeptide and expression cassette.
This locus encodes a sialyltransferase.
An appropriate sialyltransferase is used to add a modified sialic acid.
Transgenic animals harboring one or more integrated copies of a sialyltransferase transgene can be constructed.
The sialyltransferase activity may be determined by known methods.
The isolation and identification of the sialyltransferase genes were extremely difficult.
Sialyltransferase can be used in combination with additional glycosyltransferases.
Purification of sialyltransferase.
The sialyltransferase polypeptide can further comprise a signal peptide.
The bound fusión protein was assayed for sialyltransferase activity using a variety of acceptor substrates.
Each sialyltransferase is specific for a particular sugar nucleotide donor substrate.
The signal sequence directs translocation of the sialyltransferase polypeptide through the cell membrane.
The sialyltransferase polypeptides of the invention can also be produced as fusión proteins.
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The concentrated media was assayed for sialyltransferase activity using oligosaccharides as acceptor substrates.
Sialyltransferase protein was immunoprecipitated from media and cell lysales at each time point.
The complete reading frame for porcine sialyltransferase was obtained from two overlapping clones.
This substrate can be transferred enzymatically to glycans present of asialo proteins using a sialyltransferase enzyme.
Three members of the sialyltransferase family of glycosyltransferases have been cloned.
Sequence operably linked to the DNA encoding sialyltransferase.
In an embodiment, the sialyltransferase is a human sialyltransferase.
In conclusion expression of FSH together with sialyltransferase in PER.
Alternatively, unglycosylated sialyltransferase is produced in recombinant prokaryotic cell culture.
Other glycosyltransferases can be used, such as a sialyltransferase.
C6 cells in combination with a sialyltransferase to achieve highly sialylated isoforms of rhAAT.
Therefore, mass spectrometry was employed to provide amino acid sequence of sialyltransferase.
Cells expressing the FLAG-tagged sialyltransferase are labeled in red.
Preferably, the sialyltransferase polypeptide only comprises the sialyltransferase active domain.
In some embodiments, the invention provides sialyltransferase polypeptides.
Preferably, the encoded sialyltransferase is a mammalian sialyltransferase, more preferably a human sialyltransferase.
Following the glycoconjugation or remodeling, the peptide is optionally re-sialylated using a sialyltransferase.
For example, one can use a combination of sialyltransferase and galactosyltransferases.
The sialyltransferase polypeptides can be expressed intracellularly, or can be secreted from the cell.
For example, one can use a combination of a sialyltransferase and a galactosyltransferase.
A typical sialyltransferase and its recombinant expression product is obtained according to the following protocol,.
Positive pools of 5 were then used to isolate individual clones expressing sialyltransferase activity.
Briefly, glycosyltransferases such as sialyltransferase can be utilized for the construction of specific glycosidic linkages.
The method of claim 8, further comprising the step of purifying the tagged sialyltransferase protein.
Preferably, said nucleic acid encoding the sialyltransferase is integrated into the genome of said cell.
The terminal N - acetyllactosamine motif of this hexasaccharide is an acceptor for sialyltransferase.
In one preferred embodiment, a nucleic acid encoding a sialyltransferase is isolated by routine cloning methods.
An exemplary sialyltransferase of use in the methods of the invention is ST3Gal3.
The N-terminal amino acid sequence of porcine sialyltransferase was determined.
Figure 1, Sialyltransferase manufacturing process, cell culture and harvest.
According to the present invention, the ST6GalNAcI sialyltransferase polypeptide is expressed in a CHO cell.
This confirms that the cDNA of clone ST3N-1 is indeed that of the sialyltransferase.
The method according to claim 1, wherein the sialyltransferase polypeptide consists of the sialyltransferase active domain.
Fig . 6 depicts test results showing that the soluble sp-ST retains sialyltransferase activity.
The tagged sialyltransferase protein of claim 1, wherein the sialyltransferase polypeptide is SEQ ID NO, 4.
