Examples of 'site-specific mutagenesis' in a sentence
Meaning of "site-specific mutagenesis"
site-specific mutagenesis - a technique used in genetic engineering to introduce specific changes or mutations into a particular gene or DNA sequence at a targeted location
How to use "site-specific mutagenesis" in a sentence
Basic
Advanced
site-specific mutagenesis
Site-specific mutagenesis was used to demonstrate this fact.
This is conveniently done by site-specific mutagenesis.
Techniques for site-specific mutagenesis are well known in the art.
Sequences of the oligonucleotides used as primers in site-specific mutagenesis.
The technique of site-specific mutagenesis is well known in the art.
Modification of the nucleic acid sequence may be by site-specific mutagenesis.
Site-specific mutagenesis can be carried out using commercially available kits.
The substitutions were made using standard site-specific mutagenesis procedures.
Structure-guided site-specific mutagenesis represents a powerful tool for the dissection and engineering of protein-ligand interactions.
Such a gene is most readily prepared by site-specific mutagenesis of a bGH gene.
Site-specific mutagenesis was carried out essentially as described by Zoller et al.
Biological equivalents of a polypeptide can also be prepared using site-specific mutagenesis.
Optionally, the sequence may be mutagenized by site-specific mutagenesis to provide functional analogs thereof.
Amino acid substitutions, additions or deletions are preferably introduced by site-specific mutagenesis.
Optionally, the sequence can be mutagenized by site-specific mutagenesis to provide functional analogs thereof.
See also
They may be removed by any convenient conventional genetic engineering technique, suitably by site-specific mutagenesis.
Alternatively, oligonucleotide-directed site-specific mutagenesis procedures can be employed to introduce a desired mutation.
Modifications to such promoter regions may be made by random, or site-specific mutagenesis procedures.
This is easily accomplished by site-specific mutagenesis and similar methods known in the art.
The linker may couple with a cysteine which is the product of site-specific mutagenesis.
Such mutations may be generated using site-specific mutagenesis techniques or a PCR-based strategy.
These modified sequences can be prepared using well known methods such as site-specific mutagenesis.
Such mutant DNAs can be obtained by site-specific mutagenesis using PCR or the like.
This can be readily achieved through the use of routine recombinant technology, particularly site-specific mutagenesis.
The globin may be altered at the gene level by site-specific mutagenesis using well-known procedures.
For example, site-specific mutagenesis is often used to alter a specific portion of a DNA molecule.
For example, a variety of methods such as site-specific mutagenesis may be employed.
By site-specific mutagenesis.
Modifications to such segments may be made by random, or site-specific mutagenesis procedures.
Site-specific mutagenesis was conducted by a mutagenesis kit commercially available ( BIO-RAD ).
Another option is inactivation of N-glycosylation sites by site-specific mutagenesis.
Essentially, the construction involved site-specific mutagenesis of the protein C coding sequence.
Table . Sequences of the oligonucleotides used as primers in site-specific mutagenesis.
In general, the technique of site-specific mutagenesis is well known in the art.
Alterations in the DNA sequence encoding t-PA are made by site-specific mutagenesis.
The site-specific mutagenesis was carried out as disclosed by Zoller and Smith ( cited above ).
Modification of the DNA sequence may be by site-specific mutagenesis.
Example 1 Site-Specific Mutagenesis of the cDNA Coding for Phl p 5 Allergen.
The introduction of these codons may be accomplished by site-specific mutagenesis or the PCR.
Site-specific mutagenesis of this binding region brought about a significant decrease in BH promoter activity ( FIG . 19A ).
Conveniently, PEG can be attached to a chosen position in the linker by site-specific mutagenesis.
The XYL1 gene has been subjected to site-specific mutagenesis to reduce the XR-affinity for NADPH.
The polymerase chain reaction ( PCR ) can be used to perform site-specific mutagenesis.
Once the hGH is cloned, site-specific mutagenesis Carter et al ., Nucl.
For example, the polymerase chain reaction ( PCR ) can be used to perform site-specific mutagenesis.
Modifications may be made by random, or site-specific mutagenesis procedures.
The Kpn I site was formed by use of standard site-specific mutagenesis.
C The Cys729 Ala null allele was created by site-specific mutagenesis.
You'll also be interested in:
Examples of using Mutagenesis
Show more
Mutagenesis kit according to the supplierʼs instructions
Other forms of chemical mutagenesis are also available
Mutagenesis may be performed by any suitable method
Examples of using Site-specific
Show more
Limited site-specific physical system data
Jurisdictions may determine site-specific objectives
Site-specific background levels of radioactivity if known
