Examples of 'site-specific recombination' in a sentence
Meaning of "site-specific recombination"
Site-specific recombination refers to a genetic process where DNA strand exchange occurs at specific recognition sites, resulting in rearrangement of genetic material within a genome
How to use "site-specific recombination" in a sentence
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site-specific recombination
In some embodiments, the polynucleotide is integrated vía site-specific recombination.
Site-specific recombination can be used to accomplish recursive sequence recombination.
The selection marker gene is preferably flanked by site-specific recombination sequences.
Beta recombinase catalyzes site-specific recombination in transiently transfected mammalian cells.
In some embodiments, a nucleotide sequence comprises one or more site-specific recombination sites.
A further variant to the site-specific recombination system is to use non-homologous recombination sites.
Alternatively, the desired nucleotide sequence can be inserted by homologous recombination or site-specific recombination.
And analysed for its ability to catalyse site-specific recombination between loxP sites.
Site-specific recombination systems have been identified in several prokaryotic and lower eukaryotic organisms.
Re-transformed plants were analyzed to demonstrate site-specific recombination at the loxP sites.
In another embodiment, the site-specific recombination and endonuclease approaches described above can be combined.
In some embodiments, a nucleic acid sequence comprises one or more site-specific recombination sites.
Some site-specific recombination systems use only one protein to act on its target site.
A recombinase which recognizes the non-identical recombination sites and catalyzes site-specific recombination is required.
Background, site-specific recombination is used to precisely remove heterologous DNAs from their chromosomal locations.
See also
In specific embodiments, the insert polynucleotide comprises a nucleic acid flanked with site-specific recombination target sequences.
In one embodiment, the site-specific recombination site is located downstream of the plant regulatory element.
The promoter and ATG codon is provided at the selected site-specific recombination site.
A number of conserved site-specific recombination systems have been described in both prokaryotic and eukaryotic organisms.
The methods result in the efficient targeted genomic integration of DNA by site-specific recombination.
Cre recombinase catalyzes the site-specific recombination of an intervening target sequence located between loxP sequences.
The present invention provides a method for producing site-specific recombination of DNA in yeast.
The site-specific recombination leaves behind a recombination site which disrupts expression of the himA gene.
Gene excision frequencies by Xer site-specific recombination at dif sites.
Said site-specific recombination preferably takes place on DNA level.
The bacteriophage recombinase Cre catalyzes site-specific recombination between two lox sites.
Site-specific recombination is a process of DNA cleavage and religation mediated by an enzyme recombinase.
This approach is utilized in the site-specific recombination method described in Example 5.
Site-specific recombination has been used for removing auxiliary sequences from T-DNA regions.
Figure 3 schematically illustrates the integration of DNA into a chromosome by double site-specific recombination.
The site-specific recombination leaves behind a recombination site which disrupts expression of the GFOR enzyme.
Alternatively, said one replicon may be RNA formed by transcription following said site-specific recombination.
Conservative site-specific recombination can reversibly rearrange DNA.
The " scar " is produced upon deletion of the selectable cassette by site-specific recombination.
These recombinases could mediate site-specific recombination events in both E. coli and mammalian cells.
Protein toxin genes ; and a single internal resolution site, derived from the site-specific recombination event.
The IRS site-specific recombination site contains four DNA binding motifs for the recombinase TnpI.
Tn3 and related transposable elements, site-specific recombination and transposition.
Regulation of site-specific recombination by site-specific recombinase / nuclear receptor fusión proteins.
In another embodiment, a combination of these uses of site-specific recombination for counter-selection can be employed.
The site-specific recombination system of yeast ( Zygosaccharomyces rouxii ) pSR1 system H.
With ' recombination site ' is meant either site-specific recombination sites or transposon border sequences.
Site-specific recombination may then be catalysed by Cre-recombinase, also obtainable from coliphage P1.
The protein, designated FLP protein, catalyzes site-specific recombination events.
IRAP-mediated site-specific recombination in chromatin-associated targets.
Only this fraction ( 1/3 ) that contain both FLP and FRT had the potential for site-specific recombination.
A method for producing site-specific recombination of DNA in plant cells, comprising,.
Gateway ® technology, which uses lambda phage-based site-specific recombination Landy, A.
The site-specific recombination sequences are advantageously loxP sequences ( SEQ ID NO, 5 ) of Cre recombinase.
The oncogenes are combined with the site-specific recombination system ( R / RS ).
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Examples of using Site-specific
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Limited site-specific physical system data
Jurisdictions may determine site-specific objectives
Site-specific background levels of radioactivity if known
