Examples of 'site-specific recombinase' in a sentence
Meaning of "site-specific recombinase"
site-specific recombinase: A site-specific recombinase is a type of enzyme that catalyzes the recombination of DNA at specific target sites, allowing for precise genetic modifications in biological research and applications
How to use "site-specific recombinase" in a sentence
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site-specific recombinase
Insertion of a conditional allele flanked by site-specific recombinase target sequences ; or.
The site-specific recombinase gene may be constitutively or transiently expressed, or may be inducible.
In one case, the construct comprises a site-specific recombinase recognition site.
The site-specific recombinase may be a naturally occurring recombinase or an active fragment derivative thereof.
In one aspect, the construct comprises a site-specific recombinase recognition site.
Site-specific recombinase activity can be used to simplify and target transgenes introduced into plants.
In one embodiment, the construct comprises a site-specific recombinase recognition site.
The site-specific recombinase will catalyze a recombination reaction only between two site - specific recombination sequences.
Said polypeptide has a segment having an enzymatic activity of a site-specific recombinase.
A second recognition sequence for a site-specific recombinase positioned 3 ′ with respect to the transcription termination sequence.
In one embodiment, the targeting vector comprises a site-specific recombinase gene.
Recently, a site-specific recombinase has been purified from the Streptomyces bacteriophage.
A combination of homologous recombination and site-specific recombinase systems may be used.
The site-specific recombinase recognition site may be the FRT site from yeast.
The problem would be fully overcome using an exclusively intramolecular site-specific recombinase.
See also
Effecting production of the site-specific recombinase in said Agrobacterium strain.
One manner of removing these random insertions is to utilize a site-specific recombinase system.
CRE site-specific recombinase for deletion of plastid DNA sequences.
In a preferred embodiment, the recognition sequences for a site-specific recombinase are LoxP sequences.
Second, a site-specific recombinase at the end of this process is induced.
One way of avoiding these random insertions is to utilize a site-specific recombinase system.
However, the site-specific recombinase recognition sites are dif - like sites.
This problem would be overcome if an exclusively intramolecular site-specific recombinase can be used.
In particular, the site-specific recombinase recognition sites may be dif - like sites.
These methods are useful for transforming a plant cell with a site-specific recombinase expression cassette.
Recognition sites for a site-specific recombinase ( SSR ) immediately flank the antibiotic resistance gene.
The polynucleotide according to claim 1, wherein the recognition sequences for a site-specific recombinase are LoxP sequences.
In one embodiment, the site-specific recombinase gene encodes a Cre recombinase.
The gene alteration can introduce a recombinase into the mutant mammal, such as a site-specific recombinase.
With ' recombinase ' is meant either a site-specific recombinase or a transposase.
In the present invention, a " site-specific recombination sequence " refers to a recombination site for a site-specific recombinase.
A conditional allele flanked by site-specific recombinase target sequences ; or.
A specific excision sequence is a DNA sequence that is recognized by a site-specific recombinase.
A conditional allele flanked by site-specific recombinase target sequences ; and.
For example, in one embodiment, counter-selection can be accomplished by an inducible site-specific recombinase ( SSR ).
In one aspect, the protein is a site-specific recombinase as described herein.
In various aspects, the activator gene encodes a site-specific recombinase.
The gene encoding a site-specific recombinase ( SSR ) that recognizes the SSRTs must be inducible.
In one embodiment, the large targeting vector ( LTVEC ) comprises a site-specific recombinase gene.
FLPy encodes the protein, FLP, a site-specific recombinase acting at defined FLP-recognition targets, FRTs.
Cre, The Cre recombinase is a site-specific recombinase.
The method of claim 28, wherein said first site-specific recombinase comprises an active fragment of Cre.
The method of claim 13, wherein expression of the site-specific recombinase is limited to a particular tissue.
In a specific embodiment, the site-specific recombinase is Cre.
Therefore the site-specificity of the most efficient site-specific recombinase namely, Cre has been altered.
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