Examples of 'strand cdna synthesis' in a sentence
Meaning of "strand cdna synthesis"
Strand cDNA synthesis: This is a technical term related to molecular biology and genetics. It refers to the process of creating a complementary DNA (cDNA) strand from an RNA template. It is an essential technique in genetic research for studying gene expression and regulation
How to use "strand cdna synthesis" in a sentence
Basic
Advanced
strand cdna synthesis
A special oligodeoxynucleotide is used for priming of first strand cDNA synthesis.
Second strand cDNA synthesis may then proceed using conventional techniques.
Synthesizing first strand cDNA from mRNA by employing conventionally available first strand cDNA synthesis kit.
Thermocycling can induce first strand cDNA synthesis from the RNA transcript target.
MoMLV reverse transcriptase is used for first strand cDNA synthesis.
Second strand cDNA synthesis was performed by the method of Gubler and Hoffman.
The reverse transcription reaction was performed using the RevertAid First Strand cDNA Synthesis kit.
Second strand cDNA synthesis was performed with RNAse H treatment followed by random priming.
In one method, mRNA is isolated, and first strand cDNA synthesis is carried out.
Second strand cDNA synthesis was performed according to the manufacturer 's instructions.
CDNA was generated according to'the following protocol, First strand cDNA synthesis.
Preferably, the first strand cDNA synthesis is synthesised by a reverse transcriptase.
A 3 ' gene specific primer was employed for first strand cDNA synthesis.
First strand cDNA synthesis is performed as described in Example 1.
The 40 µl reaction mixture is used for second strand cDNA synthesis.
See also
First strand cDNA synthesis was carried out using oligodt primers ( SuperScript Choice GibCo BRL ).
RT-PCR was performed using the SUPERSCRIPT preamplification system for first strand cDNA synthesis ( Gibco BRL ).
First strand cDNA synthesis was carried out using SuperScript II RT ( Invitrogen ).
P1 is used to prime first strand cDNA synthesis from human B-cell polyA+ RNA.
The first strand cDNA synthesis was carried out using Promega 's Reverse Transcription system kit.
Thaw the Second Strand cDNA Synthesis reagents ( yellow ).
First strand cDNA synthesis was primed by an oligo ( dT ) primer.
Superscript III first strand cDNA synthesis kit ( InVitrogen ™ ) used according to the manufacturer 's instructions.
First strand cDNA synthesis was performed according to manufacturer 's instructions ( Invitrogen ).
After first strand cDNA synthesis the reaction mix was diluted 1/10.
First strand cDNA synthesis kit and other molecular biology reagents were from Promega ( Madison, WI ).
First strand cDNA synthesis was performed using the Superscript RT-PCR system ( Gibco BRL ).
The first strand cDNA synthesis products were then purified using a Centricon-30 concentrator device ( Amicon ).
First strand cDNA synthesis was primed using the lyase gene-specific antisense oligonucleotide A7204.
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