Examples of 'strand displacement' in a sentence
Meaning of "strand displacement"
strand displacement: Refers to a molecular biology technique where one strand of a DNA or RNA molecule is replaced or exchanged by another complementary strand
How to use "strand displacement" in a sentence
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strand displacement
The enzyme possesses strand displacement activity.
Strand displacement amplification using thermophilic enzymes.
Nucleic acid amplification by strand displacement amplification.
The strand displacement activity is preferably provided by the polymerase.
Synthesis is continual and involves a strand displacement mechanism.
Isothermal strand displacement nucleic acid amplification.
In such embodiments sequencing may proceed by strand displacement.
Strand displacement is necessary to result in synthesis of multiple copies of a target sequence.
Homopyrimidine PNAs are used for strand displacement in homopurine targets.
Strand displacement was measured using two different combinations of complementary primers.
This stopping is also referred to as a prevention of strand displacement herein.
Other polymerases having strand displacement activity may also be used.
We have also used electron microscopy to confirm strand displacement.
Cascades of strand displacement reactions can be used for either computational or structural purposes.
The samples were then ready for strand displacement assays.
See also
The strand displacement replication process obviates the need for increased temperature.
A useful polymerase enzyme can have strand displacement activity.
Strand displacement allows for the synthesis of multiple tandem copies of the circular vector.
Extension of the TeloPrimer is achieved by a strand displacement or strand degradation reaction.
Crowding agents are preferably present in step e as to decrease the risk of strand displacement.
Strand displacement assay involves the radiolabeling of DNA duplexes.
The annealed samples are kept on ice until strand displacement reaction.
Alternatively, strand displacement conditions can be employed.
A target nucleic acid is amplified enzymatically while avoiding strand displacement.
Strand displacement was ascertained by the following type of experiments,.
In certain embodiments the polymerase may have strand displacement activity.
Probing of strand displacement complex with,.
A target nucleic acid is amplified enzymaticaily while avoiding strand displacement.
Alternatively, polymerases with strand displacement activity may be used.
Isothermal methods which do not involve an RNA synthesis step proceed vÃa strand displacement.
Figure 4 demonstrates the strand displacement of five different polymerases.
In embodiments of this aspect the polymerase may have strand displacement activity.
Thus, strand displacement and nucleic acid synthesis are carried out in one step.
White filled arrows indicate partially displaced strand displacement stop products.
Strand displacement can be facilitated through the use of a strand displacement factor, such as helicase.
Different assay formats can be used in conjunction with the strand displacement assay as described.
Occasionally, strand displacement factors are used in conjunction with the polymerase to effectuate strand displacement.
Some also stiffen the sugar phosphate backbone further inhibiting strand displacement by the polymerase.
The polymerases with strand displacement activity can be either heat-unstable or heat-stable.
Such polymerases are referred to herein as strand displacement DNA polymerases.
Assays carried out using strand displacement conditions can be preformed under essentially-constant environmental conditions.
SDA conditions are those conditions compatible with strand displacement amplification.
A preferred strand displacement replication process is rolling circle amplification ( RCA ).
Replication of the RCN genome occurs through the strand displacement method.
However, the semiconservative, strand displacement mechanisms proposed by Spies et al.
Discussion The preceding examples describe an improved form of the strand displacement assay.
Strand displacement amplification ( SDA ) is also carried out in accordance with known techniques.
The step is catalyzed by a DNA polymerase catalyzing complementary strand synthesis which includes strand displacement.
These include transcription-mediated amplification, strand displacement amplification and ligase chain reaction.
The method of claim 52 wherein said amplification reaction is strand displacement.
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