Examples of 'strand cdna' in a sentence
Meaning of "strand cdna"
strand cdna - in molecular biology, this refers to the single-stranded complementary DNA synthesized from a messenger RNA template
How to use "strand cdna" in a sentence
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strand cdna
First strand cDNA was synthesized from mRNA as described above.
The mRNA is reverse transcribed to generate first strand cDNA.
First strand cDNA was prepared from the patient specimens.
A special oligodeoxynucleotide is used for priming of first strand cDNA synthesis.
First strand cDNA was prepared from a panel of lymphoma patient specimens.
Reverse transcriptase and deoxynucleotides are added to allow synthesis of the first strand cDNA.
Second strand cDNA synthesis may then proceed using conventional techniques.
During this procedure the mRNA is reverse transcribed to produce first strand cDNA.
The length of labeled first strand cDNA was determined by agarose gel electrophoresis.
MoMLV reverse transcriptase is used for first strand cDNA synthesis.
First strand cDNA is a particularly useful original template for the present methods.
The purified RNA is used as a template to prepare double strand cDNA.
Synthesizing first strand cDNA from mRNA by employing conventionally available first strand cDNA synthesis kit.
Expression levels were determined by PCR amplification of serial dilutions of first strand cDNA.
First strand cDNA was used directly for PCR amplification.
See also
RNA is isolated from candidate hybridomas and used to synthesize first strand cDNA.
First strand cDNA was prepared using the total RNA as template.
PCR amplification of inhibitor cDNA fragments from the salivary gland first strand cDNA.
First strand cDNA was synthesized using random oligonucleotides and SuperScript II.
Thermocycling can induce first strand cDNA synthesis from the RNA transcript target.
First strand cDNA was prepared using identical amounts of total RNA from each subject.
These were then used in a PCR to amplify DNA from first strand cDNA.
The synthesis of first strand cDNA was as described in the Stratagene protocol above.
Preparation of Triatoma pallidipennis salivary gland RNA and synthesis of first strand cDNA.
First strand cDNA was synthesized on RNA isolated from a variety of cell types.
The reverse transcription reaction was performed using the RevertAid First Strand cDNA Synthesis kit.
The resulting first strand cDNA was amplified in using two HGV specific primers.
This allowed linear amplification of the 2nd strand cDNA.
The first strand cDNA was synthesized by following the BRL cDNA synthesis protocol.
Artemis cDNA cloning and genomic amplification First strand cDNA was synthesized from fibroblast RNA.
First strand cDNA is synthesized from the total RNA using oligo dT as the primer.
In one method, mRNA is isolated, and first strand cDNA synthesis is carried out.
First strand cDNA was synthesized from the total RNA using oligo dT as the primer.
CDNA was generated according to'the following protocol, First strand cDNA synthesis.
The single strand cDNA was tailed by following the protocol of Boehringer Mannheim tailing kit.
Oligo-dT was used for reverse transcription of viral RNA into first strand cDNA.
Second strand cDNA synthesis was performed by the method of Gubler and Hoffman.
Second strand synthesis was carried out using T4 DNA polymerase, first strand cDNA as template.
Second strand cDNA synthesis was performed with RNAse H treatment followed by random priming.
And using a DNA polymerase I, double strand cDNA was synthesized.
Preferably, the first strand cDNA synthesis is synthesised by a reverse transcriptase.
A 3 ' gene specific primer was employed for first strand cDNA synthesis.
Second strand cDNA synthesis was performed according to the manufacturer 's instructions.
The 40 µl reaction mixture is used for second strand cDNA synthesis.
For the first strand cDNA synthesis, primers with an antisense sequence are used.
The reaction was set up with 1st strand cDNA and 1uM oligonucleotide.
First strand cDNA is synthesized by using oligo ( dT ) and avian myeloblastosis virus reverse transcriptase.
The reaction is set up with 1st strand cDNA and 1uM oligonucleotide.
First strand cDNA was synthesized by using oligo ( dT ) and avian myeloblastosis virus reverse transcriptase.
Labelled cRNA ( 1 µg ) from the patient was used to generate first strand cDNA.
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Examples of using Cdna
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The cDNA is divided into two portions
The orientation of bikunin cDNA was confirmed by sequencing
A cDNA was also isolated from a human library
