Examples of 'subclone' in a sentence
Meaning of "subclone"
subclone (noun): In biology, a 'subclone' refers to a genetically distinct subgroup of cells that are derived from a primary cell line. It is often used in the context of cell culture and genetic research to describe specific cell populations
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- A clone or descendant of a mutant occurring in a previous clone
- A partial segment of DNA, cloven from a previously-cloned segment, which is then cloned into another vector
- To reclone part of a previously-cloned DNA segment into a new vector
How to use "subclone" in a sentence
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subclone
No subclone consisted of only one cell type.
Ten plates were made per subclone.
The subclone of interest is selected using the functional complementation screen.
These phenotypes may be due to inherent properties of a given subclone or to mutation.
The dominant subclone was further analyzed under a variety of reaction conditions.
This is particularly useful when it is necessary to subclone larger transgenes into a vector.
The best subclone of each chimeric transfectoma was isolated and analyzed for antibody production.
Standard molecular biology techniques could be used to subclone this gene into a mammalian expression vector.
All subsequent deletions and insertions of EGF repeats were performed in this subclone.
The possibility to directly subclone the completed constructs into vectors appropriate for eukaryotic gene expression.
The sequence of both DNA strands for each subclone was determined.
Thus the need to subclone DNA as an independent step is eliminated.
A composite sequence of the Rb gene then can be assembled from these individual subclone sequences.
The DNA insert of this clone was used to subclone the gene into a plasmid vector.
The cartridges are insertable into cloning vehicles that are used to clone and subclone DNA.
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Several subclone supernatatants were screened by ELISA.
A restriction map of this subclone is given in Figure 4.
However, a subclone containing only the tellurite resistance determinant displayed no reduced uptake.
Phage clone SLRD-A was used to subclone fragments for sequence analysis.
Subclone the purified DNA band into pZERO1 at EcoRV site.
However, attempts to subclone larger fragments were unsuccessful.
Plasmid restriction mapping, Southern hybridization, and PCR analysis confirmed the identity of each subclone.
This deletion subclone was digested with Bam HI restriction endonuclease.
Using pBluescript as the cloning vector, it was possible to subclone the 25 kb fragment.
The resulting subclone was designated pDPTpgi2.
The sequence of the exon-containing region of each subclone is shown in Figure 4a-c.
Table 8 shows the subclone identification nomenclature and monikers.
Of these four colonies, 3T3 gE clone 9 was used to isolate a subclone having a high gE expression.
Therefore, these subclone supernatants were tested on protease pretreated xenografts only.
Figure 3 shows the nucleotide sequences for human TRAIN receptor from a subclone of lambda gt10 cDNA.
This subclone was drug-marked so that it would not survive in HAT medium.
Phage clone SLRD-A was used to subclone the tfr genes for sequence analysis.
First, a subclone containing a unique LTR was constructed and named pUC LTR.
These intermediate clones were used to subclone DEN-1 virus-specific cDNA fragments.
An M13 subclone of the HIV gag region was used as the target nucleic acid template.
In the present study, the subclone Ton . Kit.
Based on the obtained subclone a gene disruption vector was generated as described for A. sojae.
Dr. Thomas ' group had supplied the Der f II cDNA as a subclone in pGEX.
PCR was used to subclone the cloned gene in frame into the expression vector pGEX 2T.
C, The retroviral titer of a single G418 resistant targeted subclone was monitored over time.
These are used to subclone PCR-generated fragments of the Rop1 subunits into two different vectors.
The E. coli DH5 alpha strain was used to subclone and make constructs.
This subclone is known as pcamH3EF.
Figure 5 presents the flow chart of MDCK Subclone 1-B serum free cell bank preparation.
This subclone contained about 1.5 kb of sequences upstream from the NcoI site.
Using Gateway, one can clone subclone DNA segments for functional analysis.
The E+ subclone MZ2-MEL 4B was used as a source of DNA for preparation of a cosmid library.
An identical strategy was used to subclone the HPV 45 L1 gene into pFastBac.
The obtained subclone containing a full-length cDNA of human MTAse was designated MTAP-7.
Mouse NIH3T3 cells were infected with the virus supernatant of a G418 resistant subclone.
