Examples of 'subcloned' in a sentence
Meaning of "subcloned"
subcloned (verb) - This refers to the process of creating a cloned copy of a previously cloned gene or DNA fragment in genetic engineering or molecular biology research
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- simple past tense and past participle of subclone
How to use "subcloned" in a sentence
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subcloned
Probes were generated from subcloned restriction fragments.
Regions subcloned are indicated above the plasmid.
Ten of these twelve clones were further subcloned.
Positive colonies were subcloned twice by limiting dilution.
This band was cut out and subcloned.
These cDNAs have been subcloned and are being sequenced.
All positive clones were selected and subcloned.
These hybridomas were subcloned to monoclonality by limiting dilution.
These clones were expanded and subcloned.
Hybridomas of interest were subcloned using limiting dilution.
Cells are assayed for IgG production and subcloned.
The construct is thereafter subcloned into a binary vector.
Positive clones were isolated and subcloned.
An SstI fragment was subcloned into the pSK vector.
A relatively stable high producer clone was selected and subcloned.
See also
Selected blocking hybridomas were subcloned by limiting dilution.
Clones secreting high levels of chimeric antibody were further subcloned.
The positive clones were subcloned and sequenced.
Is subcloned and subsequently inserted into pKapl digested with SmaL.
This hybridoma was later subcloned by a limiting dilution technique.
Transformants are identified by conventional methods and may be subcloned if desired.
Positive clones were subcloned by limiting dilution and rescreened.
The cells were then subcloned.
Amplified cDNAs were subcloned and sequenced as described above.
Hybridomas producing specific HuMab IgG were subcloned and expanded.
The cDNA was subcloned into pBlsc and sequenced.
Some of the fragments were further subcloned and sequenced.
The cDNA was subcloned into pBS and sequenced.
The two largest cDNA inserts were subcloned and sequenced.
Both fragments were subcloned in the same orientation generating pborIH.
Selected hybridomas were subcloned twice.
The remainder were subcloned and their isotype was determined.
The second coding exon was sequenced using these subcloned fragments.
Transfected cell lines were subcloned according to standard techniques.
A panel of hybridomas was selected and the hybridomas were subcloned and expanded.
The culture was subcloned by limiting dilution.
For this purpose the ubiquitin promoter had to be first subcloned.
Positive clones were subcloned several times to guarantee purity.
This fragment was subcloned.
So this is subcloned into a plasmid.
Single clones were isolated manually and further subcloned by serial dilution.
Transcribed cDNAs are subcloned into appropriate vectors to establish a cDNA library.
Cells in the positive wells were further subcloned by limiting dilution.
Clones may be subcloned by limiting dilution procedures and grown by standard methods.
Recombinants thus identified were subcloned and further characterized.
The subcloned lines are retested for specific antibody activity to fungal components.
Stable cell lines were subcloned from initial transformation.
The two products of expression were visualized and their respective ORFs were subcloned.
The contruct is thereafter subcloned into a binary vector.
Positive wells were identified by screening the cells harvested and then subcloned once.
