Examples of 'subcloning' in a sentence
Meaning of "subcloning"
Subcloning is the process of cloning a DNA fragment that has already been cloned
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- present participle of subclone
- The production of a subclone
How to use "subcloning" in a sentence
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subcloning
This subcloning is repeated to yield monoclonal hybridomas.
These clones were selected for further subcloning.
Subcloning was done using limited cell dilution method.
It has been modified slightly to facilitate genetic subcloning.
The subcloning was carried out in two orientations.
Different approaches to subcloning methods are shown.
Subcloning and sequencing was performed as described above.
Both clones were selected for subcloning by limiting dilution.
Repeated subcloning using limiting dilution was done.
These two clones were subjected to another round of subcloning.
Subcloning and sequencing identified the muc gene.
A principal reason for subcloning is to fuse protein domains.
Subcloning and construction of expression vectors.
It must have been generated during subcloning.
Successful subcloning was confirmed by restriction analysis.
See also
The antibody sequences were then ready for subcloning.
Such subcloning techniques are well known in the art.
This inhibition is useful in cloning and subcloning procedures.
This subcloning process is repeated to obtain a uniform hybridoma.
Expression levels often can be increased several fold by subcloning.
Subcloning and sequencing of the inhibitor cDNA fragments.
The method for the subcloning will be described hereinafter.
Subcloning of tTF was performed in two stages.
These two plasmids are constructed to facilitate subsequent subcloning experiments.
The subcloning process went as follows.
Hybridoma cloning and subcloning.
Subcloning is carried out by standard procedures known in the art.
This transfer is called subcloning.
Subcloning into a plasmid vector and characterization of the transformant.
Restriction mapping revealed several convenient sites for subcloning.
Subcloning and sequencing of cDNA.
Components of the homologous recombination cloning and subcloning methods.
Subcloning was accomplished by limiting dilution.
This was consistent with results obtained by the subcloning experiments.
The results of subcloning and sequencing of human genomic fragments were as follows.
Fusion of the polynucleotides may be accomplished by subcloning in intermediate vectors.
Subcloning is performed to ensure that a clonal population of cells has been identified.
Positive supernatants were screened in this way at each subcloning of hybridomas.
Subcloning was performed as follows.
The specificity of these bands was confirmed by subcloning and sequencing.
Subcloning and sequencing.
A variety of restriction enzyme sites for simplified subcloning are present.
Subcloning is used to select for the optimal cell line properties.
Diagram of subcloning strategy.
Subcloning was done in two steps.
Appropriate orientations for subsequent subcloning were determined by restriction digest analysis.
The resulting fragment has artificial ends for convenient subcloning.
The restriction sites used in the subcloning are EcoRI and XhoI.
The region of the acid phosphatase gene was further specified by subcloning.
Subsequent subcloning of these subclones should produce clones that are monoclonal in nature.
