Examples of 'ligation product' in a sentence
Meaning of "ligation product"
ligation product: In molecular biology, this term denotes the result of joining DNA fragments together using a DNA ligase enzyme. It refers to the DNA molecule formed after the ligation process
How to use "ligation product" in a sentence
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ligation product
The ligation product is then sequence verified.
Numbers above lanes indicate relative amounts of ligation product.
The ligation product was clearly revealed.
The oligonucleotides are subsequently ligated to form a single ligation product.
The ligation product is digested with BamHI and with HinDIII.
A hybridization method may also be used to assay the ligation product.
Said first ligation product is subsequently immobilised to the surface or a matrix.
The ligation should produce a significant or detectable amount of ligation product.
No ligation product was detected.
The reaction product of the ligation is referred to as the first ligation product.
The ligation product was transformed into NovaBlue cells and yielded multiple colonies.
The intermediate spontaneously rearranges to generate the full length ligation product.
The ligation product can then be detected by mass spectrometry and compared to a known value.
None of the other ligases produced any detectable ligation product under isothermal conditions.
This circularized ligation product is suitable for rolling circle amplification and subsequent sequencing.
See also
Taq DNA ligase reaction did not yield a detectable ligation product.
The ligation product was transformed into NovaBlue cells and several colonies were selected for screening.
PCR amplification of the ligation product.
The third ligation product ultimately comprises the target sequence as depicted in Fig.
The resulting stable probe / anchor ligation product is fluorescently detected.
The ligation product was used to transform DH5a competent cells.
In certain embodiments, an amplification method may be used to assay the ligation product.
Digestion of the ligation product with Not I confirmed that this site had been destroyed.
In certain embodiments, a standard PCR method may also be used to assay the ligation product.
Integrating the ligation product into an expression vector ; and.
In certain embodiments, quantitative end-point PCR may also be used to assay the ligation product.
Optionally, all ligations extend the ligation product in the same direction.
The ligation product was inserted in bacteria, yielding roughly three million independent transformants.
The major peak corresponds to the photochemical ligation product of CVU G with Tag1 PsoCVU.
The ligation product was digested with SalI and ligated to SalI digested pV2.
Performing a restriction digest of the bound ligation product of step ( h ),.
The ligation product was transformed by electroporation into an electrocompetent maintenance strain of E. coli.
Next, competent cells of E . coli DH5a were transformed with the ligation product.
The ligation product was amplified in E. coli and the plasmid was purified by standard methods.
An Escherichia coli HB101 strain was transformed using the ligation product.
The ligation product was digested with Xho 1 prior to transformation to eliminate parental plasmid.
At 45 °C, the amount of correct size ligation product detected was diminished.
The ligation product was subcloned into XL1 cells ; resulting transformants were screened by sequencing.
Competent TG1 bacteria ( CaCl 2 technique ) are transformed with 5 ul of ligation product.
The ligation product was transformed into DH5a competent cells and plated onto LB+ ampicillian plates.
After each ligation of the genes, the ligation product is introduced into E. coli NM522.
The ligation product is transformed to E . coli host.
Figures 3A-3C shows results of a Real-time PCR experiment to detect ligation product.
The ligation product was then transformed into E. coli by electroporation.
Following ligation, the ligation product was transformed into DH5a Max Efficiency cells.
The ligation product was used to transform electrocompetent E . coli cells.
In step 4, the first ligation product is cut with a type IIS restriction enzyme.
The ligation product was amplified by transformation of E. coli and plasmid DNA isolation.
More of the correct size ligation product was detected at ligation temperatures of 37 °C and 40 °C.
The ligation product was then used to transform the E . coli bacterial strain DH5 a.
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Examples of using Product
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We certify that the product indicated hereafter
Product serial number displayed on the product
This report is the product of a wide collaboration
