Examples of 'ligation products' in a sentence
Meaning of "ligation products"
ligation products - in biology, refers to the DNA molecules formed by joining two ends of DNA fragments
How to use "ligation products" in a sentence
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ligation products
The ligation products are propagated and harvested.
This would otherwise result in false ligation products.
The ligation products were transformed into bacteria.
Larger amounts of template yielded ligation products with intense signal.
Immobilized ligation products are detected vía their detectable label.
Said bacteria are transformed by electroporation with the purified ligation products.
The packaged ligation products were plated.
Ligation products were then purified by polyacrylamide gel electrophoresis.
The fixed sequence oligonucleotides are directly or indirectly ligated to create ligation products.
The resulting ligation products are incorporated into expression vectors.
The present invention also contemplates compositions of grafting polymers onto the ligation products.
The respective ligation products are in principle further ligated oligonucleotides.
Coli DH5a are transformed with the ligation products.
Full length ligation products are chemically identical to proteins produced by cell free synthesis.
Correct orientation of the resulting ligation products was confirmed by DNA sequencing.
See also
Ligation products are then quantified by PCR.
Several means of detecting PCR amplified ligation products can be employed as described below.
Ligation products were used to transform E.
Amplifying the contiguous ligation products to create amplification products ; and.
Ligation products were detected using an automated IMx ® analyzer as described above.
Multiple bands were observed, indicating the presence of different ligation products.
The performance of the ligation products generated by photochemical crosslinking methods was also evaluated.
Unreacted ligation probes, or target-independent ligation products are then washed away.
The obtained ligation products were amplified by PCR.
Ligating the hybridized oligonucleotides to create contiguous ligation products complementary to the loci ;.
Alternatively, the ligation products can be separated by size and detected.
Advantageously, the ligation reactions have fast reaction rates and produce ligation products in high yield.
Amplifying the ligation products using the universal primer sites to create amplicons ;.
In this embodiment, the primers used to amplify the ligation products are differentially labeled.
Ligation products are only observed when 3 ′ phosphates were present on the labeled antisense siRNA.
These lower the efficiency of the uptake of circularised, productive ligation products in E . coli.
Preferably though, all ligation products formed with all target sequences are analysed simultaneously.
Alternatively, recombinant plasmids were obtained by electroporation of above mentioned ligation products into D10HB cells.
Amplifying the contiguous ligation products using the universal primer regions to create amplification products ;.
The ligated DNA constituted the desired plasmid pPS55 and other related ligation products.
Ligation products were detected as described in Example 1.
Following universal PCR, the extension products of the original ligation products are detected and quantified.
The ligation products were ligated to vNotI / tk vaccinia virus DNA.
Amplification of the ligation products by PCR,.
The ligation products are isolated from Nusieve / agarose gel as above.
In some embodiments, the ditags are concatamerized to produce ligation products containing from 2 to 200 ditags.
Ligation products were prepared using ligation probesets designed to detect 30 SNP loci.
Coli DH10 cells were transformed with the ligation products.
The ligation products were transformed in E . coli cells and transformed clones were selected.
The bacterial strain JM109 was transformed with the ligation products.
Ligation products were transformed, screened, and sequenced through the PCR region as stated above.
The ligated DNA constituted the desired plasmid mOW390 along with other ligation products.
The ligation products were transformed into competent cells from the TA cloning kit ( Invitrogen ).
The ligated DNA constituted the desired plasmid pOG0299 along with other ligation products.
The ligation products were used to transform E. coli.
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Tubal ligation was performed as previously informed consented
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